糖基化改性玉米醇溶蛋白膜的性能及硬胶囊体外释放分析

为了探究改性对玉米醇溶蛋白膜的性能影响,明确制备硬胶囊后的体外释放规律。该研究采用葡萄糖对玉米醇溶蛋白进行湿法糖基化改性,对糖基化改性产物的机械性能、阻湿性、阻氧性、阻油性和肠溶性进行研究。结果表明,改性后玉米醇溶蛋白膜的抗拉强度为34.06 MPa,相比未改性zein膜4.67 MPa有明显提高(P0.05);改性后玉米醇溶蛋白的吸水率在24 h达到最大,为84.98%;zein-glu膜的过氧化值为0.43 g/100 g,较zein膜的过氧化值0.49 g/100 g低;zein-glu膜的水蒸气透过率在达到平稳时降至7.89×10~(-8) g·m/(m~2·d·Pa);zein-glu膜的透油系数为0,阻油性与市售保鲜膜相当;胶囊释放以罗丹明B作为胶囊填充内容物,结果表明由zein-glu制备的胶囊具有肠溶性,模拟体外释放的拟合数学模型决定系数R~2为0.800,该模型通过卡方检验、相关系数检验和t统计量的显著性检验。研究结果可为玉米醇溶蛋白的改性、成膜机制和肠溶性释放提供理论参考。     

CT小肠成像和胶囊内镜检查在不明原因消化道出血诊断中的临床价值研究

目的 探讨CT小肠成像(CTE)和胶囊内镜(CE)在不明原因消化道出血(OGIB)诊断中的作用及其价值。方法 比较研究CTE和CE在40例OGIB患者中检查结果,并最终应用手术病理结果与之进行对照。结果 CTE的诊断阳性率为45.0％(18/40),而CE的诊断阳性率高达70.0％(28/40),两者结果差异有统计学意义(P＜0.05)。而CE和CTE两者的联合诊断阳性率高达72.5％(29/40),与单纯进行CE检查相比,其结果差异无统计学意义(P＞0.05);而同单纯行CTE检查相比,其结果差异有统计学意义(P＜0.05)。结论 对OGIB的诊断,单纯行CE检查的诊断阳性率显著高于单纯行CTE检查(P＜0.05),两者联合检查的诊断阳性率较CE略有提高,但差异无统计学意义(P＞0.05),但鉴于CE和CTE具有互补性,在临床实践中对OGIB同时进行CE和CTE检查是有必要的。     

基于卷积块注意力胶囊网络的小样本水稻害虫识别

针对真实复杂背景下小样本水稻害虫识别模型泛化能力弱,易受复杂背景干扰以及重要特征表达能力不强等问题,提出了 一种基于卷积块注意力胶囊网络的小样本水稻害虫识别方法.采用数据增强的方法扩充数据,以提高模型的泛化能力同时预防过拟合;利用GrabCut算法去除图像中的复杂背景,减小复杂背景对水稻害虫识别的干扰;将空间注意机制和...     

苏黄止咳胶囊治疗风邪犯肺型感冒后咳嗽疗效观察

目的 观察苏黄止咳胶囊治疗风邪犯肺型感冒后咳嗽的临床疗效。方法 将感冒后咳嗽的患者63例,随机分成治疗组32例和对照组31例。对照组口服复方甘草片治疗,治疗组口服中成药苏黄止咳胶囊治疗,观察两组患者的咳嗽等症状缓解情况及治疗效果。结果 两组患者治疗前和治疗后咳嗽积分的组内比较以及治疗后组间的比较,差异均有统计学意义（P<0.05）;治疗组的临床总有效率达93.8%,对照组总有效率为74.2%,两组间差异有统计学意义（P<0.05）。结论 苏黄止咳胶囊治疗风邪犯肺型感冒后咳嗽,能明显缓解咳嗽等临床症状,具有很好的临床疗效,适合门诊应用。     

气相色谱-质谱法测定十味香鹿胶囊挥发油的化学成分

采用气相色谱-质谱联用法对十味香鹿胶囊的挥发油成分进行分析。采用2010年版《中国药典》挥发油测定法对十味香鹿胶囊挥发油进行提取,以毛细管柱进行分析,面积归一化法测定百分含量,并对其化学成分进行鉴定。色谱柱为Agilent19091M-436型弹性石英毛细管色谱柱(0.25mm×30m,0.25μm);程序升温:60℃,以10℃/min升至280℃,保持12min。进样口温度为280℃。质谱条件:EI离子源,电子能量70eV,离子源温度230℃;扫描范围20~500amu。共检出33个化合物,鉴定出其中的22个化学成分,相对含量超过5%的成分有棕榈酸(54.107%)、顺-9-十八烯酸甲酯(8.092%)、反-9-十八碳烯酸(7.285%)、(Z,Z)-9,12-十八碳二烯酸(6.026%)、9-反十六碳烯酸甲酯(5.456%),同时鉴定出具有镇痛作用的丁香酚甲醚(0.172%)。十味香鹿胶囊中挥发油成分主要包含棕榈酸、烯酸以及烯酸甲酯类成分等。     

禽多杀性巴氏杆菌C48-3株hexABC基因缺失株的构建及其生物学特性分析

为研究荚膜在禽多杀性巴氏杆菌(Pasteurella multocida)致病过程中的作用,本研究利用同源重组基因敲除技术构建禽P.multocida C48-3株荚膜多糖输出蛋白基因hexABC的缺失突变株,并以小鼠为动物模型检测荚膜缺陷对细菌毒力的影响。PCR、RT-PCR和DNA测序结果均表明253 bp的hexC基因下游序列、798 bp的hexB基因全序列和290 bp的hexA基因上游序列完全被四环素抗性基因替代,表明构建了基因敲除突变株C48-3ΔhexABC。电镜观察结果表明突变株荚膜合成能力缺失,对小鼠的致病性试验结果显示突变株毒力基本丧失。本研究获得的无荚膜突变株为进一步研究禽P.multocida的致病机理奠定基础。     

Evaluation of a wireless ambulatory capsule (SmartPill®) to measure gastrointestinal tract pH, luminal pressure and temperature, and transit time in ponies

Reasons for performing study: This study investigated the use of a wireless ambulatory capsule (WAC; SmartPill^® pH.p GI Monitoring System) to determine WAC‐gastric emptying time (GET) in ponies. Objectives: To measure WAC‐GET and compare it to those findings with GET assessed by nuclear scintigraphy (S‐GET). Hypothesis: WAC‐GET will be slower than S‐GET, but will be significantly correlated. Methods: Seven healthy adult mixed‐breed pony mares were used in this study. Feed was withheld for 12 h prior to the WAC administration. After administration, a complete‐feed diet was fed to allow the WAC to pass into the stomach. Luminal pH, temperature and pressure were collected by a modified receiver secured to the pony. Once the pH reached a value of ≥8.0, it was determined that gastric emptying had occurred, and ponies were fed grass hay. After 5 days, data were downloaded and analysed using proprietary software. During the second period of the study, after at least 2 weeks, 4 of the ponies underwent a standard S‐GET test. Results: The WAC was successfully administered, and data were collected from all ponies. The mean percentage of data packets collected by the receiver was 84.9 ± 3.51% (range 66.8–95.1%). Mean WAC‐GET was 7.38 h (range 0.15–46.65 h). Mean gastric pH was 4.75 (range 2.07–6.99). Mean small intestinal transit time was 4.6 h. The mean pH for the small intestine was 8.0. The mean S‐GET time (in hours) when 10% of the radioactive feed is present in the stomach (T‐90%) was 2.3 h. The S‐GET did not correlate significantly with the WAC‐GET. Conclusions and potential relevance: The WAC was safely administered to ponies, and data were collected using a modified receiver. The WAC‐GET varied considerably between ponies, but was ≤3 h in 5 of the 6 ponies. The WAC used in this study provided a noninvasive technique that produced novel information about the pony gastrointestinal tract, but owing to the substantial variability in GET values and long transit time it may not be a reliable clinical tool at this time.     

清脂胶囊质量标准研究

目的:建立清脂胶囊的质量控制方法。方法:对清脂胶囊中白术、白芍、柴胡进行薄层色谱鉴别;采用HPLC法对芍药苷进行含量测定。结果:薄层色谱斑点清晰,阴性对照无干扰;芍药苷进样量在0.104-1.040μg范围内线性关系良好(r=0.998 5),平均回收率为98.92%,RSD为0.61%(n=9)。结论:本研究建立的TLC和HPLC法专属性强、准确度高、重复性好,可用于清脂胶囊的质量控制。     

紫外可见光分光光度法测定莽吉柿胶囊中总双苯吡酮的含量

建立一种测定莽吉柿胶囊中总双苯吡酮含量的方法。以α-倒捻子素为标准品,以NaNO2-Al(NO3)3-NaOH为显色系统,采用紫外可见光分光光度法在370nm测量波长测定莽吉柿胶囊中总双苯吡酮的含量。紫外可见光分光光度法符合精密度、准确度、线性(r=0.999)的基本要求,加样回收率为100.38%,RSD为2.49%。该方法简便、准确、灵敏度高、重现性好,可用于莽吉柿胶囊中总双苯吡酮的含量测定。     

RP—HPLC—UVD法测定莽吉柿胶囊中alpha—mangostin的含量

建立一种测定莽吉柿胶囊中α-倒捻子素（alpha—mangostin）含量的方法。采用反相高效液相色谱-紫外检测法测定。色谱条件：Diamonsil^TM C18色谱柱（250mm×4．6mm,5μm）,流动相为甲醇-水（83：17）,流速为1．0mL／min,柱温：25℃,紫外检测波长：317nm。在选定的色谱条件下,反相高效液相色谱-紫外检测法符合精密度、准确度、线性（r=0．9995）的基本要求,加样回收率为100．9％,RSD为1．82％。该方法简便、准确、灵敏度高、重现性好,可用于莽吉柿胶囊中alpha—mangostin的含量测定。